![]() Stereology can be ‘model-based’: points within a frame are counted to estimate the ‘volume density’ (Vv) of a structure, and intercepts are counted to assess the ‘surface density’ (Sv). Moreover, the analyses based on digital images should consider the same format and the same size (pixels) to all pictures allowing a comparison between groups. In histological sections, the ‘caps’ of the objects that have been sectioned tangentially are lost when chemical agents remove the paraffin distorting the analysis. Correction factors may be necessary for the retraction and compression of the tissues. Quantitative variables can be ‘continuous’ or ‘discrete.' In this text, the term ‘morphometry’ is used to design a simpler procedure for measuring structures with a ‘ruler.' The term ‘stereology’ is used in estimating quantities in the 3-dimensional space analyzing 2-D cut sections. Quantitative results are analyzed with statistics and should be based on a well-defined sample. ![]() The quantitative morphology contributes to making the studies less subjective and reproducible. Tips for Studies with Quantitative Morphology (Morphometry and Stereology). MANDARIM-DE-LACERDA, Carlos Alberto and DEL SOL, Mariano.
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